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Image Search Results
Journal: Biomolecules
Article Title: TIPE2 Induced the Proliferation, Survival, and Migration of Lung Cancer Cells Through Modulation of Akt/mTOR/NF-κB Signaling Cascade
doi: 10.3390/biom9120836
Figure Lengend Snippet: Knockout of TIPE2 in lung cancer cells and its effect on different cancer hall marks. ( A ) Western blot analysis showing the expression of TIPE2 in NCIH460 lung cancer cells after CRISPR/Cas9 knockout (Left panel); Effect of TIPE2 knockout on the viability of NCIH460 lung cancer cells as analyzed by MTT assay (Right Panel); ( B ) Colony formation assay showing decreased clonogenic potential of lung cancer cells after knockout of TIPE2 (Left panel). Graphical representation of decreased clonogenic potential of TIPE2 knockout cells in terms of survival fraction (Right panel). ( C ) Cell migration was detected with the help of wound healing assay. Images were taken at 10× magnification at 0 and 24 h (Left panel). Graphical representation of the decreased migration potential of TIPE2 knockout cells compared to CRISPR/Cas9 scramble (right panel). ( D ) Effect of TIPE2 knockout on the progression of cell cycle in lung cancer cells analyzed through flow cytometry using PI/RNase solution (Left panel). Graphical representation of the effect of TIPE2 knockout on the cell cycle progression of lung cancer cells (Right panel). Data are represented as mean ± SE, * denotes p value < 0.05.
Article Snippet:
Techniques: Knock-Out, Western Blot, Expressing, CRISPR, MTT Assay, Colony Assay, Migration, Wound Healing Assay, Flow Cytometry