Journal: Cancer Management and Research

Article Title:

Integrative gene expression profiling reveals that dysregulated triple microRNAs confer paclitaxel resistance in non-small cell lung cancer via co-targeting MAPT

doi: 10.2147/cmar.s215427

Figure Lengend Snippet: Figure 1 Identification of paclitaxel-resistant NSCLC cells. Notes: (A) Human NSCLC cells (H460_Parental and H460_TaxR) treated with indicated concentrations of paclitaxel for 72 h were subjected to cell viability assay. (B, C) H460_Parental and H460_TaxR cells were grown in triplicates in the absence or presence of indicated concentrations of paclitaxel for 2–3 weeks. The pictures and numbers of the cell colonies were obtained by the QuantiOne software of Fluor-STM Multimager. (D, E) H460_Parental and H460_TaxR cells were treated with indicated concentrations of paclitaxel for 24 hrs. Cells were collected and subjected to Western blot analyses of PARP, Casp-3 or β-actin (D), or apoptotic-ELISA (E). Abbreviations: F-PARP, full length of poly(ADP-ribose) polymerase; C-PARP, cleaved PARP; Pro-Casp-3, Caspase-3; C-Casp-3, cleaved caspase-3; ELISA, enzyme-linked immunosorbent assay.

Article Snippet: Cells and cell culture Human large-cell lung carcinoma cell line H460 was obtained from American Type Culture Collection (Manassas, VA, USA) and maintained in RPMI1640 medium supplemented with 10% fetal bovine serum (FBS).

Techniques: Viability Assay, Software, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Cancer Management and Research

Article Title:

Integrative gene expression profiling reveals that dysregulated triple microRNAs confer paclitaxel resistance in non-small cell lung cancer via co-targeting MAPT

doi: 10.2147/cmar.s215427

Figure Lengend Snippet: Figure 4 MAPT-subnetwork in paclitaxel-resistant NSCLC cells. Notes: (A) qRT-PCR analysis of mRNA level of MAPT in H460_Parental and H460_TaxR. (B) qRT-PCR analysis of miRNAs levels of miR-766-3p, miR-362-3p, and miR-6507- 3p in H460_Parental and H460_TaxR. (C) Diagram of targeting sequences of miR-766-3p, miR-362-3p, and miR-6507-3p in 3ʹ-UTR of MAPT mRNA. (D) Diagram of constructed MAPT-subnetwork in paclitaxel-resistant non-small cell lung cancer cells. Green, downregulated; Red, upregulated. Abbreviations: MAPT, microtubule-associated protein tau; qRT-PCR, quantitative reverse transcriptase PCR; 3ʹ-UTR, 3ʹ-untranslated region.

Article Snippet: Cells and cell culture Human large-cell lung carcinoma cell line H460 was obtained from American Type Culture Collection (Manassas, VA, USA) and maintained in RPMI1640 medium supplemented with 10% fetal bovine serum (FBS).

Techniques: Quantitative RT-PCR, Construct, Reverse Transcription

Journal: Biomolecules

Article Title: TIPE2 Induced the Proliferation, Survival, and Migration of Lung Cancer Cells Through Modulation of Akt/mTOR/NF-κB Signaling Cascade

doi: 10.3390/biom9120836

Figure Lengend Snippet: Knockout of TIPE2 in lung cancer cells and its effect on different cancer hall marks. ( A ) Western blot analysis showing the expression of TIPE2 in NCIH460 lung cancer cells after CRISPR/Cas9 knockout (Left panel); Effect of TIPE2 knockout on the viability of NCIH460 lung cancer cells as analyzed by MTT assay (Right Panel); ( B ) Colony formation assay showing decreased clonogenic potential of lung cancer cells after knockout of TIPE2 (Left panel). Graphical representation of decreased clonogenic potential of TIPE2 knockout cells in terms of survival fraction (Right panel). ( C ) Cell migration was detected with the help of wound healing assay. Images were taken at 10× magnification at 0 and 24 h (Left panel). Graphical representation of the decreased migration potential of TIPE2 knockout cells compared to CRISPR/Cas9 scramble (right panel). ( D ) Effect of TIPE2 knockout on the progression of cell cycle in lung cancer cells analyzed through flow cytometry using PI/RNase solution (Left panel). Graphical representation of the effect of TIPE2 knockout on the cell cycle progression of lung cancer cells (Right panel). Data are represented as mean ± SE, * denotes p value < 0.05.

Article Snippet: NCIH460 human NSCLC cells were procured from National Centre for Cell Science (NCCS), Pune, India.

Techniques: Knock-Out, Western Blot, Expressing, CRISPR, MTT Assay, Colony Assay, Migration, Wound Healing Assay, Flow Cytometry